Quantitative analysis of gene expression using PCR
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United States of America Patent
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Apr 22, 2003
Grant Date -
N/A
app pub date -
Feb 1, 2001
filing date -
Jul 21, 1998
priority date (Note) -
Expired
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Abstract
The invention provides an improved method for quantitation of two target genes in a simultaneous PCR, without the need to determine limiting primer concentrations for both targets. In particular, the invention provides a method of quantitation of expression of a first and a second target protein-encoding nucleic acids using a PCR based assay in which amplification of nucleic acid is detected by release of a reporter fluorescent signal which is measured as a nomialized reporter value ('deltaRn').
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