Multiple sorbent cartridges for solid phase extraction
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United States of America Patent
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-
Apr 1, 2003
Grant Date -
N/A
app pub date -
Nov 9, 2000
filing date -
Nov 12, 1999
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Methods and apparatus are disclosed which are useful for detecting the presence of various pesticides, including aryl pyrazoles such as fipronil. Pesticide residues are extracted from a variety of samples, including soil and/or water samples using techniques well known to those of skill in the art. The samples are then concentrated, and transferred to an solid phase extraction cartridge. The SPE cartridge includes activated charcoal, silica gel, magnesium-silica gel and an amino-functional silica sorbent, or suitable equivalents thereof. After cleanup, the eluate containing the analyte of interest can be concentrated to a standard sample size and the amount of pesticide residues quantitated. In a preferred embodiment, the column chromatography materials are assembled in a solid phase extraction cartridge with frits between the chromatography materials. This facilitates the use of the cartridge in standard automated analytical equipment, for example, robotic equipment.
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