Method for creating polynucleotide and polypeptide sequences
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United States of America Patent
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Mar 25, 2003
Grant Date -
Sep 26, 2002
app pub date -
Dec 4, 1998
filing date -
Dec 8, 1997
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Abstract
The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.
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