Target amplification of nucleic acid with mutant RNA polymerase
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United States of America Patent
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Mar 11, 2003
Grant Date -
N/A
app pub date -
May 30, 2000
filing date -
Jun 2, 1999
priority date (Note) -
Expired
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Abstract
This invention provides for a novel amplification procedure for nucleic acid. The method uses a mutant RNA polymerase designed to transcribe deoxynucleotides. Using primers that bind to target nucleic acid the primers form polymerase recognition sites that permit transcription of the target in an isothermal and logrithmic manner, with the added advantage of forming multiple single stranded copies, which are readily detected by hybridization assays.
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