Method of producing activated lipase
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United States of America Patent
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Mar 4, 2003
Grant Date -
N/A
app pub date -
Oct 12, 1999
filing date -
Mar 24, 1999
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method is disclosed for activating a lipase by adding a solution of lipase in an aqueous buffer at a pH near neutrality to an organic phase, e.g., tetradecane. Under these conditions lipase is activated as a function of an organic-and-water boundary surface between the organic and water phases. The lipase that is activated in this manner remains active even after lyophilization to remove the water and the organic phase. This activated lipase efficiently catalyzes a fat reforming reaction in non-aqueous and nano-aqueous conditions.
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