Cell-free replication system and uses thereof
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United States of America Patent
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Feb 18, 2003
Grant Date -
N/A
app pub date -
Mar 9, 2000
filing date -
Sep 2, 1997
priority date (Note) -
Expired
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Abstract
The invention relates to improvements in cell-free systems for initiating DNA replication under cell cycle control. The system comprises a synchronous population of G1 nuclei which have been released from G0; and S phase cytosol. In a preferred aspect, a polypeptide is supplied to the system, wherein the polypeptide is Cdc6 and/or at least one MCM protein. The system is suitable for DNA replication assays, for example to test substances which modulate DNA replication.
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