Method of permanent fluorescent assay
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United States of America Patent
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Feb 11, 2003
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app pub date -
Mar 16, 2000
filing date -
Mar 17, 1999
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Abstract
A method for performing a peroxidase-based assay by reacting a peroxidase enzyme with a soluble organic p-hydroxyphenyl-containing compound having the formula: ##STR1## wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from --OH, --COOH, --NH.sub.2, and --SH, and wherein R is selected from --OH, --COOH, --NH.sub.2, and --CH.sub.3 ; and converting the soluble organic p-hydroxyphenyl-containing compound into a stable insoluble product which becomes highly fluorescent after illumination, upon excitation.
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