Mathematical analysis for the estimation of changes in the level of gene expression
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
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Dec 31, 2002
Grant Date -
N/A
app pub date -
May 24, 2000
filing date -
May 24, 2000
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
The present invention relates to a highly accurate and reproducible mathematically-based methodology for quantifying the levels of differential gene expression from microassay protocols. Specifically, the present invention provides a simple deductive approach, grounded in a Bayesian framework, to circumvent the heuristic-based limitation of previous methodologies. Rather than seeking a point-estimate of the fold-change of the level of gene expression, the present invention utilizes the derivation of mathematical formula to determine the a posteriori distribution of all the fold-changes of differential gene expression which may be inferred from the given measurements. From this a posteriori distribution the following information may be obtained: (i) an estimator for the fold-change of the level of gene expression; (ii) confidence limits for the fold-change, at any given confidence level; and (iii) a P-value for assessing the statistical significance of change. The present invention also possesses the advantage that fold-change estimates and confidence limits may even be assigned to signal pairs where both signals are zero or negative, without resorting to heuristic thresholds.
First Claim
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