Methods of initiating embryogenic cultures in plants
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United States of America Patent
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Dec 10, 2002
Grant Date -
N/A
app pub date -
Oct 11, 2000
filing date -
Jun 19, 2000
priority date (Note) -
Expired
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Abstract
The present invention provides methods for initiating embryogenic cultures of plants. The methods include the use of novel media compositions and elevated atmospheric pressure treatments to improve the frequency of embryogenic culture initiation. The methods are well suited for initiating embryogenic cultures in recalcitrant conifer varieties. The method is also well suited for producing somatic embryos that can be further cultured to produce large numbers of plants. Further, the invention provides novel methods that may be used to enhance somatic embryogenesis in a broad range of species.
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