Method for preparing template DNA from processed vegetable food, which is feasible for amplification of DNA region by PCR method
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United States of America Patent
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Dec 10, 2002
Grant Date -
Mar 28, 2002
app pub date -
Jan 31, 2000
filing date -
Nov 26, 1999
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Disclosed is a method for preparing a template DNA from a processed vegetable food, comprising the steps of: extracting a crude DNA fraction from a processed vegetable food subjected to at least one of a high temperature treatment, a high temperature grinding treatment, a high pressure treatment and a fermentation treatment, and optionally subjected to defatting; and performing a DNA fractionation treatment of the crude DNA fraction by a polyethylene glycol precipitation method and/or a polyacrylamide gel electrophoresis method. Also disclosed is a method for detecting a gene of a plant in a processed vegetable food derived from the plant by a PCR method in which such a template DNA is used. The processed vegetable food may be an oil source seed.
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