Compounds having energy transfer function and method for DNA base sequencing by using the same
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United States of America Patent
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Nov 19, 2002
Grant Date -
N/A
app pub date -
Sep 17, 1999
filing date -
Jul 11, 1997
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Expired
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Abstract
Disclosed are compounds having two kinds of reporters that can be a donor and an acceptor for energy transfer, for example, fluorescent groups, and having a 2', 3'-dideoxyribonucleotide residue or a 3'-deoxyribonucleotide residue. These compounds can be used as terminators for the chain terminator method. The two kinds of reporters are arranged with a distance sufficient for causing energy transfer from the donor to the acceptor. Also disclosed are methods for determining DNA sequences based on the chain terminator method wherein the chain termination reaction is performed by using the above terminators. Also disclosed are compounds having two kinds of reporters that can be a donor and an acceptor for energy transfer, which can be used as a primer or an initiator in methods for determining DNA sequences utilizing the chain terminator method, and methods for determining DNA sequences utilizing the compounds.
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