Fluorescence polarization-based homogeneous assay for fumonisin determination in grains

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United States of America Patent

PATENT NO 6482601
SERIAL NO

09659663

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Abstract

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A homogeneous assay for determining the fumonisin content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with a solvent. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to fumonisin. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating fumonisin to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The fumonisin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of fumonisin solutions of known concentration.

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Patent Owner(s)

Patent OwnerAddress
DIACHEMIX LLC223 NORTH WATER STREET SUITE 500 MILWAUKEE WI 53202

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Jolley, Michael E Round Lake, IL 27 635
Nasir, Mohammad Sarwar Grayslake, IL 6 7

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