Method for producing nucleic acid polymers
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United States of America Patent
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Oct 29, 2002
Grant Date -
N/A
app pub date -
May 12, 2000
filing date -
Aug 22, 1997
priority date (Note) -
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Abstract
The conventional synthesis of nucleic acid polymers from several oligonucleotides comprises several cycles of intermediate product synthesis, purification of the intermediate products and synthesis of a full length product (up to a maximum length of approximately 1000 nucleotides). The novel method shall be carried out in one reaction step and result in nucleic acid polymers of more than 1000 nucleotides. According to the invention, two or more linkable oligonucleotides are provided that in a continuous arrangement and after linkage can form a primary strand, and one or more non-linkable oligonucleotides are provided, each of the non-linkable oligonucleotides comprising two adjoining regions, the first of which is complementary to the 3' end of a linkable oligonucleotide and the second of which is complementary to the 5' end of a further linkable oligonucleotide. The linkable oligonucleotides are hybridized with the complementary regions of the non-linkable oligonucleotides and then linked enzymatically, chemically or photochemically. The method is suited for the de novo synthesis of long nucleic acid chains.
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