Hydrolytic enzyme substrates and assay method
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United States of America Patent
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Sep 24, 2002
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N/A
app pub date -
Nov 3, 2000
filing date -
Nov 5, 1999
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Expired
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Abstract
Compounds of the invention have the structure D--L--A*, where L is a linking moiety that maintains D and A in a configuration compatible with FRET, D is a fluorescent donor moiety capable of causing an acceptor moiety to fluoresce by FRET, and A* is a fluorescent acceptor moiety capable of accepting energy from the donor moiety by FRET and fluorescing only after cleavage of one or more labile groups to provide acceptor A. These compounds are useful as fluorescent substrates for detection of enzymes in vivo.
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