Selection method for producing recombinant baculovirus

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United States of America Patent

PATENT NO 6428960
SERIAL NO

09702181

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Abstract

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The present invention provides a novel method for producing recombinant baculoviruses expressing genes of interest by inserting a selectable marker gene, specifically the puromycin resistance (pac) gene, adjacent to the gene of interest into the baculovirus genome, and selecting for viruses that express the selectable marker. Selection of the recombinant virus in the presence of a selection condition, such as puromycin-containing cell culture media, simplifies virus production by eliminating the need for cloning, and testing the virus for gene expression. Such expression systems are readily adapted to an automated method for expression foreign genetic material in a high throughput manner.

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Patent Owner(s)

Patent OwnerAddress
REGENTS OF THE UNIVERSITY OF CALIFORNIA THE1111 FRANKLIN STREET 12TH FLOOR A CALIFORNIA CORPORATION OAKLAND CA USA 94607-5200
ONYX PHARMACEUTICALS INCONE AMGEN CENTER DRIVE THOUSAND OAKS CA 91320-1799

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Albala, Joanna Stockton, CA 2 112
Clark, Robin Benecia, CA 40 970
Franke, Ken Gaithersburg, MD 3 110
McConnell, Ian Danville, CA 5 123

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