Method of chromatographically purifying or fractionating, respectively, von Willebrand factor from a VWF-containing starting material
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United States of America Patent
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Jul 2, 2002
Grant Date -
N/A
app pub date -
Oct 21, 1999
filing date -
Feb 4, 1997
priority date (Note) -
Expired
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Abstract
Disclosed is a method of chromatographically purifying or fractionating, respectively, von Willebrand factor (vWF) from a vWF-containing starting material, comprising the following steps: adsorbing the vWF from the starting material on avid collagen immobilized on a carrier, separating the non-adsorbed portion and, optionally, washing the carrier, eluting the vWF from immobilized collagen, and recovering the purified vWF, as well as a pharmaceutical preparation comprising biologically active vWF which is bound to collagen in a stable manner.
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