Methods for identifying enzyme inhibitors
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United States of America Patent
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Jun 18, 2002
Grant Date -
N/A
app pub date -
Mar 2, 2001
filing date -
Jul 6, 1998
priority date (Note) -
Expired
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Abstract
A method for identifying an inhibitor of dehydroquinate synthase (DHQS) and/or dehydroquinase (DQ), which method comprises: i) contacting a test substance with DHQS and a substrate for DHQS and contacting the resulting reaction mixture with DQ, or contacting the test substance with DQ and a substrate for DQ; and ii) contacting the resulting reaction mixture with dehydroshikimate dehydratase (DHSD); and iii) determining whether the test substance inhibits the activity of DHQS or DQ.
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