Method of determining the enzymatic activity of the blood coagulation factor XIII using purified fibrin monomer as a substrate
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United States of America Patent
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Jun 18, 2002
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N/A
app pub date -
Feb 17, 1999
filing date -
Jun 18, 1997
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Expired
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Abstract
The present invention relates to a method of determining the enzymatic activity of blood coagulation factor XIII by using purified fibrin monomer as a substrate of this enzyme. The enzymatic activity is determined by detecting the degree of cross-linking of fibrin monomer formed by the blood coagulation factor XIII and the fibrin monomer free of blood coagulation factor XIII is obtained by washing the preparation with citric acid solution. The present method can be effectively used for the validation of the other enzymatic assay methods for the blood coagulation factor XIII as well as the studies for the characterization of the blood coagulation factor XIII.
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