Temperature dependent ligand facilitated purification of proteins
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United States of America Patent
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Apr 30, 2002
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N/A
app pub date -
May 5, 1999
filing date -
Sep 4, 1998
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Abstract
The invention concerns the use of a coiled coil protein which is capable of forming a homodimer which dissociates into monomers on change of temperature, in a method of isolating a ligand or desired protein from a sample. A method of isolating a ligand which binds to the coiled coil protein is described as well as methods of isolating a desired protein which is tagged with a coiled coil protein or a ligand which binds to a coiled coil protein, or is tagged with a coiled coil protein monomer.
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