Enhanced catalyzed reporter deposition
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United States of America Patent
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Apr 16, 2002
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N/A
app pub date -
Nov 5, 1999
filing date -
Nov 9, 1998
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Abstract
A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent conmprising an inorganic salt, an organic compound having the formula ##STR1## wherein when X is B(OH).sub.2, Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from --OH, --COOH, --NH.sub.2, and --SH, and wherein R is selected from --OH, --COOH, --NH.sub.2, and --CH.sub.3 ; or mixtures of the inorganic salt and the organic compound. A compound having the formula: ##STR2## wherein R.sup.1 is --CH.sub.2 OH, --COOH, and n is 1-8.
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