Method of DNA sequencing
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United States of America Patent
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Apr 2, 2002
Grant Date -
N/A
app pub date -
Nov 10, 1999
filing date -
Jul 7, 1997
priority date (Note) -
Expired
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Abstract
Disclosed are methods for determining DNA nucleotide sequences comprising reacting ribonucleoside 5'-triphosphates and 3'-dNTP derivatives in the presence of an RNA polymerase modified so as to enhance its ability for incorporating the 3'-dNTP derivatives and a DNA fragment containing a promoter sequence for the RNA polymerase to obtain a nucleic acid transcription product, separating the resulting nucleic acid transcription product, and determining a nucleic acid sequence from the resulting separated fraction. These methods can produce a transcription product of a long chain and afford more accurate sequence data where fluctuation of signals from labeled deoxyribonucleotides is reduced.
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