Method of coupling ligands to a solid phase in acidic solution and anionic surfactant
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United States of America Patent
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Mar 19, 2002
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N/A
app pub date -
Jun 16, 1999
filing date -
Dec 17, 1996
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Abstract
The present invention relates to a method for binding antibody or antigen molecules to solid phase. The reactive compound comprising an antibody or antigen molecule and a solide phase are formed by coupling a specific antibody or antigen molecule to a solid phase using a two-step reaction. The coupling is covalent, between the active groups of the solid phase and the antibody, a fragment thereof or the antigen molecule, in the presence of an anionic surfactant, after adding an alkaline/solution to raise the pH to the basic region.
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