FLUORESCENCE POLARIZATION- BASED DIAGNOSTIC ASSAY FOR EQUINE INFECTIOUS ANEMIA VIRUS
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United States of America Patent
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Feb 26, 2002
Grant Date -
Feb 28, 2002
app pub date -
Sep 21, 1999
filing date -
Sep 23, 1998
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Abstract
A fluorescence polarization assay for Equine Infectious Anemia Virus utilizes a short peptide reagent probe derived from a conserved immunodominant region of gp45. The probe is N-terminally labeled, preferably with 6-carboxyfluorescein, and purified by HPLC, which reacts in a homogenous assay with anti-EIAV antibodies contained in the serum of field infected horses and ponies. The assay has a sensitivity of about 90 percent with a specificity approaching 100 percent.
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