Method to separate cells that have been modified with superparamagnetic particles by ballistic transfer
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United States of America Patent
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Feb 19, 2002
Grant Date -
N/A
app pub date -
May 5, 1995
filing date -
May 9, 1994
priority date (Note) -
Expired
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Abstract
The ballistic transfer transfection technology employs a cold gas shock wave to accelerate microprojectiles that carry matter into cells by mechanical force. The present invention relates to a method that enables separation of transfected cells by co-adsorbing polynucleic acids and nanometer-sized superparamagnetic particles onto the microprojectiles. The transfected cells are rendered magnetically susceptible and can thus be separated by retaining them in a strong magnetic field. The use of the technology in a clinical context is facilitated.
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