Regulated autocrine growth of mammalian cells
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United States of America Patent
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Jan 22, 2002
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app pub date -
Jan 26, 1998
filing date -
Jul 26, 1995
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Abstract
Methods of production of desired recombinant proteins, polypeptides and peptides are disclosed which utilize mammalian host cells engineered for autonomous and regulated growth in low cost protein/serum-free culture media. Preferred host cells express insulin or an insulin-like growth factor, and/or transferrin and are engineered so that addition of an inducer (e.g., ZnCl.sub.2 +CdCl.sub.2) halts growth and simultaneously induces the expression of the desired recombinant protein, polypeptide or peptide.
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