13C,15N ,2H labeled proteins for NMR structure determinations and their preparation
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United States of America Patent
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Jan 1, 2002
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app pub date -
Mar 15, 2000
filing date -
Sep 2, 1997
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Abstract
This invention is concerned with determining the three-dimensional structure of biological macromolecules such as proteins. In particular, it is concerned with methods for rapidly determining protein structure by NMR, by providing methods for simplifying NMR spectra using labeled proteins prepared from specifically isotopically labeled amino acids having at least two isotopes of 13C, 15N and 2H in the backbone, and methods for making these labeled proteins, e.g., by cultivation of a microbial culture containing said labeled amino acids.
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