Process for fast visualization of protein
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United States of America Patent
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Nov 20, 2001
Grant Date -
N/A
app pub date -
Oct 13, 1999
filing date -
Oct 13, 1998
priority date (Note) -
Expired
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Abstract
The present invention provides an improved method for staining proteins in and/or on a solid matrix or support. Typically, a protein-containing gel or membrane may be washed in a hot solution of water for about five minutes, stained in a hot solution of COOMASSIE brilliant blue dye in dilute aqueous mineral acid for about five minutes, and then rinsed in water. The washing and/or staining steps may be performed by placing the gel in a wash and/or staining solution, respectively, heating the solution in which the gel is placed to boiling in a microwave oven, and incubating the gel in the solution for about five minutes. The entire procedure can be performed in a little over ten minutes, which represents an enormous time savings over existing methods.
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