Method for analyzing monosaccharide in a sugar composition
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United States of America Patent
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Nov 20, 2001
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N/A
app pub date -
Apr 21, 2000
filing date -
May 28, 1999
priority date (Note) -
Expired
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Abstract
A method for the determination of monosaccharides in a sugar composition by simultaneous quantitative analysis of monosaccharides constituting the sugar composition, comprising the steps of: (1) liberating sialic acid from the sugar composition with sialidase or an acid, (2) converting the released sialic acid into N-acylmannosamine with sialic acid aldolase, and (3) acid hydrolyzing the N-acylmannosamine and a sugar residue. The method allows pretreatment in a single reactor and the sugar composition including sialic acid can be obtained by a single analysis by HPLC, etc.
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