Method of producing a structured layer
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United States of America Patent
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Oct 2, 2001
Issued Date -
N/A
app pub date -
Sep 1, 1999
filing date -
Mar 4, 1997
priority date (Note) -
Expired
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Abstract
The invention relates to a method for producing a structured layer of defined functional molecules on the surface of a flat substrate, on the surface of which structures having different surface properties, at least as regards their hydrophobicity, are produced. A monolayer of a protein-containing crystalline cell surface layer (S layer) is deposited by recrystallization on said structured surface. Said S Layer binds only to those structured areas of the surface characterized by raised hydrophobicity. Alternatively, a structured S-layer may also be produced on the basis of a monolayer of an S layer deposited on a substrate by irradiating predefined sections of said layer to be structured with radiation of a predetermined intensity and energy. In the irradiated sections of the S-layer this suppresses the binding or intercalating ability of at least one surface. Functional molecules can bind to or become intercalated in the surface or the intermediate spaces of the crystal lattice, for example for use as bio-sensors.
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