Production of high titers of gibberellins, GA4 and GA7, by Gibberella fujikuroi strain LTB-1027
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United States of America Patent
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Sep 11, 2001
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N/A
app pub date -
Aug 23, 2000
filing date -
Aug 31, 1999
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Abstract
A new method of producing a mixture of gibberellins which is predominantly GA.sub.4 and GA.sub.7 but also contains GA.sub.3 has the steps of providing a seed of Gibberella fujikuroi Strain LTB-1027 or mutants derived therefrom, inoculating the seed into a culture medium rich in carbohydrate and relatively low in nitrogen, incubating the culture for at least four days, separating the Gibberella fujikuroi Strain LTB-1027 from the culture broth, and extracting the gibberellins to produce a gibberellin mixture which is at least 50% GA.sub.4 and GA.sub.7. The method produces a gibberellin mixture in which the combined titer of GA.sub.4 and GA.sub.7 exceeds 800 mg/liter. The production method also produces a gibberellin mixture with approximately equal titers of gibberellins GA.sub.4 and GA.sub.7. A variation of the method produces a gibberellin mixture which contains over 40% GA.sub.4.
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