Method of amplifying DNA fragment, apparatus for amplifying DNA fragment, method of assaying microorganisms, method of analyzing microorganisms and method of assaying contaminant
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United States of America Patent
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Sep 11, 2001
Grant Date -
N/A
app pub date -
Mar 30, 1999
filing date -
Mar 31, 1998
priority date (Note) -
Expired
status (Latency Note)
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Abstract
An apparatus for amplifying DNA fragments is formed by a support plate. A plurality of openings are formed on the upper surface of the support plate. A plurality of primers having different amplification probabilities are arranged in the plurality of openings in order of the amplification probabilities. A plurality of microorganisms contained in a microorganism flora are simultaneously amplified with all primers by a random PCR method, for obtaining an electrophoretic pattern amplified at the optimum amplification probability for each microorganism. The plurality of microorganisms contained in the microorganism flora can be discriminated by analyzing the electrophoretic pattern.
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