(1.fwdarw.3, 1.fwdarw.4)--.beta.-glucanase of enhanced stability
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United States of America Patent
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Aug 21, 2001
Issued Date -
N/A
app pub date -
Jan 11, 1996
filing date -
Jul 7, 1993
priority date (Note) -
Expired
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Abstract
This invention relates to a (1.fwdarw.3, 1.fwdarw.4)-.beta.-glucanase (glucanase EII endohydrolase) enzyme, whose amino acid sequence has been modified in order to provide an enzyme whose three-dimensional structure confers improved thermostability and/or pH stability. Specific modifications are based upon a comparison between the three-dimensional structure (1.fwdarw.3, 1.fwdarw.4)-.beta.-glucanase and that of (1.fwdarw.3)-.beta.-glucanase. The (1.fwdarw.3, 1.fwdarw.4)-.beta.-glucanase gene has been modified by site-directed mutagenesis, and modified enzymes have been expressed in E. coli. Modified sequences, DNA molecules encoding them, plasmids, expression vectors and transgenic plants are disclosed.
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