Specific binding reagent comprising a variable domain protein linked to a support or tracer
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United States of America Patent
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Aug 14, 2001
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N/A
app pub date -
Apr 12, 1994
filing date -
Dec 1, 1989
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Abstract
Small specific binding molecules, such as single variable domain antibodies (Dabs) and Fv fragments, can be coupled to solid plastics surfaces or to tracers such as enzymes by means of linkers comprising polypeptides containing from 5 to 20 amino acids and which are hydrophobic and/or contain at least one lysine residue. The coupling can be achieved without significant loss of specific binding activity. The combined Dab/linker or Fv/linker can be prepared by expression in genetically-modified organisms.
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