Mass spectrometric methods for sequencing nucleic acids
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United States of America Patent
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Jul 31, 2001
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N/A
app pub date -
Dec 15, 1997
filing date -
Dec 15, 1997
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Abstract
A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods use a modified Sanger sequencing strategy in which RNA polymerase is used to generate a set of nested RNA transcripts obtained by base-specific chain termination. These are analyzed by mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.
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