Recombinant poliovirus vector and method of preparing the same
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United States of America Patent
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Jul 3, 2001
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app pub date -
May 12, 1998
filing date -
Feb 1, 1995
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Abstract
The invention relates to a recombinant poliovirus vector and method of making the vector. The recombinant poliovirus vector comprises an RNA polymerase promoter operably linked to a poliovirus nucleic acid sequence which has been altered by removal of the CelII-SnaBI fragment and insertion of a heterologous nucleic acid sequence encoding a protein of interest. The heterologous nucleic acid sequence is flanked on both sides by internal ribosomal entry sites which allow for the expression of the encoded protein of interest and subsequent proteins encoded by the altered poliovirus nucleic acid sequence.
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