Rapid methods for identifying modifiers of cellular apoptosis activity
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United States of America Patent
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Jun 26, 2001
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N/A
app pub date -
May 10, 1999
filing date -
Jun 5, 1997
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Expired
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Abstract
The invention provides a single-well, microscale method of determining the specific apoptotic activity of a cell. The method consists of contacting a cell population of about 1.times.10.sup.5 cells for a time period of between about 30 minutes and 4 hours with a sufficient volume of medium containing an apoptotic specific diagnostic reagent and a diagnostic accessory reagent so as to cover the cell population, and determining the activity of the apoptotic specific diagnostic reagent. The invention also provides a method of identifying a compound which induces apoptosis. The invention further provides a rapid method of identifying a compound which inhibits apoptosis.
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