Method and apparatus for rapid hygiene testing
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United States of America Patent
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Apr 17, 2001
Grant Date -
N/A
app pub date -
Oct 15, 1999
filing date -
Feb 19, 1997
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A rapid detection of ATP using dried luciferase/luciferin reagent. A liquid that may include an extraction reagent is applied to the testing surface, such as a counter top. A sampling device having a handle and an absorbent tip is wiped across the testing surface. The absorbent tip absorbs the sample. The sample is then placed in a counting tube. Luciferase/luciferin reagent is immobilized on the absorbent tip of the sampling device or in the bottom of a counting tube. In either case, the liquid rehydrates the luciferase/luciferin reagent allowing ATP present in the sample to react with the luciferase. Light produced by the reaction is measured with a luminometer.
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