Expression cloning processes for the discovery characterization, and isolation of genes encoding polypeptides with a predetermined property
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United States of America Patent
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Mar 6, 2001
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app pub date -
Nov 17, 1997
filing date -
Nov 17, 1997
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Abstract
The present invention is directed to a method for detection, characterization and isolation of nucleic acids encoding proteins of a desired property, such as a particular cellular localization. The invention further provides for rapid expression of such proteins or glycoproteins in mammalian cells and for facilitated purification of the novel secreted proteins or glycoproteins. Further, the invention provides for radioactive labelling of the novel proteins or glycoproteins, for rapid identification of sites of binding including animals and for rapid production of infective viral vectors for use in gene transfer.
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