Vectorial cloning system of DNA
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United States of America Patent
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Feb 13, 2001
Grant Date -
N/A
app pub date -
Oct 5, 1999
filing date -
Dec 20, 1996
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The invention relates to a vectorial cloning system consisting of a sequence of nucleotides, containing a fusion sequence and one or several restriction endonucleases, recognition sites for restriction endonucleases cutting outside their recognition sites, in addition to containing one or several other restriction endonuclease recognition sites which can be used to clone a foreign protein as well as the sequence for the desired foreign protein, wherein the foreign protein sequence is directly located on the fusion sequence after a subsequent restriction with the restriction endonucleases, followed by religation.
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