Industrial scale process for the preparation of 2-hydroxy-4-methylbutyric acid using a nitrilase
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United States of America Patent
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Jan 30, 2001
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app pub date -
Oct 24, 1997
filing date -
Oct 25, 1996
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Abstract
This invention relates to a process for the preparation of 2-hydroxy-4-methylthiobutyric acid or the ammonium salt of 2-hydroxy-4-methylthiobutyric acid by enzymatic hydrolysis of 2-hydroxy-4-methylthiobutyronitrile, comprising: a) preparing a biological material having a nitrilase activity; b) immobilizing the biological material, c) exposing the 2-hydroxy-4-methylthiobutyronitrile to the biological material thus immobilized to obtain the ammonium salt of 2-hydroxy-4-methylthiobutyric acid; and d) optionally converting the salt obtained to the corresponding acid.
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