Electropolymerizable film, and method of making and use thereof
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United States of America Patent
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Jan 30, 2001
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app pub date -
Mar 12, 1999
filing date -
Jun 27, 1995
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Abstract
An electrode and method of preparing an electrode by electropolymerizing a film on the conductive working surface of an electrode. The electrode is modified by reductive electropolymerization of a thin film of poly[Ru(vbpy).sub.3.sup.2+ ] or poly[Ru(vbpy).sub.3.sup.2+ /vba] (vbpy=4-vinyl-4'methyl-2,2'-bipyridine and vba=p-vinylbenzoic acid) and the electrode is used for the electrochemical detection of aqueous GMP, poly[G], and surface immobilized single-stranded DNA probes. The film is formed from a co-polymer of a mediator such as Ru(vbpy).sub.3.sup.2+ and a functionalized moiety having a carboxylate group such as p-vinylbenzoic acid. A DNA probe is attached covalently to the carboxylate group via a carbodiimide reaction followed by amidation of an amino-linked single-stranded DNA. In the presence of these guanine containing moieties, a dramatic enhancement in the oxidative current for the Ru.sup.3+/2+ couple (present in the polymeric thin film) due to the catalytic oxidation of guanine is observed.
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