Reagents for labeling SH groups, process for the preparation of them and method for labeling with them
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United States of America Patent
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Jan 9, 2001
Grant Date -
N/A
app pub date -
Nov 14, 1997
filing date -
Mar 15, 1996
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Disclosed are SH-labeling reagents containing acridine compounds represented by the following formula (I): ##STR1## wherein A represents the following group: --(CH.sub.2).sub.m1 -- or --(CH.sub.2).sub.m2 --Q--(CH.sub.2).sub.n -- in which Q represents a group --S.sup.+ RX.sup.- --, a group --N.sup.+ RR.sub.1 X.sup.- -- wherein R.sub.1 represents an alkyl group having 1 to 6 carbon atoms or an aryl group, a group ##STR2## wherein R.sub.2 and R.sub.3 may be the same or different and are each independently a group --(CH.sub.2).sub.k -- (k: a number of 1 to 3) or --O(CH.sub.2 CH.sub.2 O).sub.l -- (l: a number of 1 to 3), m1 stands for a number of 1 to 6, m2 denotes a number of 0 to 2, n means a number of 1 to 2; R represents an alkyl group having 1 to 6 carbon atoms or an aryl group; and X.sup.- represents an anion, or intermediates thereof; preparation processes of the acridine compounds; and methods for labeling analytes by using the compounds.
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