Solid phase immunoassay to detect inhibitors of proteolytic enzymes
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United States of America Patent
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Dec 12, 2000
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N/A
app pub date -
Sep 23, 1996
filing date -
Mar 29, 1994
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Abstract
Solid phase immunoassay for detecting specific inhibitors of proteolytic enzymes in biological fluids, which comprises: a) contacting a tubulin peptide covalently linked to a support with a solution containing a proteolytic activity together with a protease inhibitor, b) detecting the inhibitor activity against the selected proteases by contacting the support with a solution containing a labelled monoclonal antibody which specifically recognises the free end of the tubulin peptide linked to the support.
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