Chitin beads, chitosan beads, process for preparing these beads, carrier comprising said beads, and process for preparing microsporidian spore
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United States of America Patent
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-
Dec 5, 2000
Grant Date -
N/A
app pub date -
Dec 30, 1998
filing date -
May 14, 1997
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention relates to chitin beads having a uniform and fine particle size and comprising microsporidian spores whose principal cell wall substance is chitin; chitosan beads comprising N-deacetylated chitin and having a uniform fine particle size; methods for preparing these beads; carriers comprising these beads; and a method for preparing microsporidian spores. Microsporidian spores are proliferated in insect's bodies or cultured cells to obtain chitin beads having the uniform and fine particle size and whose cell wall substance is mainly composed of chitin. The chitin as the major cell wall substance is subjected to N-deacetylation to give chitosan beads. When the insect is a larva of domesticated silkworm, microsporidian spores are proliferated by orally or percutaneously inoculating the spores into the 2nd instar domesticated silkworm larvae in a concentration of 5.times.10.sup.2 to 5.times.10.sup.8 spores/ml, then the proliferated microsporidian spores are isolated from the bodies of the grown 5th instar domesticated silkworm larvae, followed by purifying the resulting spores to give chitin beads having the uniform and fine particle size and chitosan beads can be obtained from the chitin beads in the same manner used above. These beads are used as carriers for the immobilization or encapsulation of a variety of substances such as an antibiotic and a microorganism.
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