Quiescent cell populations for nuclear transfer in the production of non-human mammals and non-human mammalian embryos
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United States of America Patent
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Nov 14, 2000
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Feb 19, 1997
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Aug 31, 1995
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Abstract
A method of reconstituting a mammalian embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more mammals. The invention is useful in the production of transgenic mammals as well as non-transgenics of high genetic merit.
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