Production of orthomyxoviruses in monkey kidney cells using protein-free media
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United States of America Patent
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Nov 14, 2000
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app pub date -
Oct 15, 1997
filing date -
Nov 10, 1994
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Abstract
Viruses from the family Orthomyxoviridae, particularly influenza virus, can grown in monkey kidney cells, particularly Vero Cells, after passaging the cells in a serum-free or protein-free medium. The use of a proteolytic enzyme, especially trypsin, also aids in the propagation of the virus. The method allows for the virus to be produced to be used in a vaccine.
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