Measuring method using whole blood sample
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United States of America Patent
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Nov 7, 2000
Grant Date -
N/A
app pub date -
Mar 29, 1996
filing date -
Jul 29, 1994
priority date (Note) -
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Abstract
A method for quantitatively measuring analyte in an undiluted whole blood sample is disclosed which involves contacting an undiluted whole blood sample comprising the analyte with magnetically-responsive particles which comprise a first partner which is coated on a magnetically-responsive particulate insoluble carrier, wherein the first partner specifically binds to the analyte to form a first complex of insoluble carrier-first partner-analyte, separating the resulting first complex from the undiluted whole blood sample, thereafter contacting the resulting separated first complex with a second partner which is labelled with a detectable marker and which specifically binds to the analyte to form a second complex of insoluble carrier-first partner-analyte-second partner, and separating the resulting second complex from unbound second partner by washing, and thereafter detecting the marker in the second complex to quantitatively measure the analyte in the undiluted whole blood sample, and wherein the initial contacting occurs for a period of time prior to an end-point of the binding reaction of the first partner and the analyte.
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