Conjugates and specific immunoassays for the methadone metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine
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United States of America Patent
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Oct 31, 2000
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Sep 24, 1998
filing date -
May 27, 1997
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Abstract
Novel chemical analogs of the methadone metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) are disclosed. The derivatives can be used for formation of EDDP-protein conjugates. The conjugates can be used in turn to raise antibodies reactive with EDDP and having a low cross-reactivity with methadone. The antibodies and EDDP-enzyme polypeptide conjugates provide the basis for specific immunoassays used in monitoring compliance with methadone treatment.
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