Mutant proteolytic enzymes and method of production
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United States of America Patent
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Oct 24, 2000
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N/A
app pub date -
Nov 26, 1997
filing date -
May 29, 1991
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Expired
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Abstract
Mutant B. lentus DSM 5483 proteases are derived by the replacement of at least one amino acid residue of the mature form of the B. lentus DSM 5483 alkaline protease. The mutant proteases are expressed by genes which are mutated by site-specific mutagenesis. The amino acid sites selected for replacement are identified by means of a computer based method which compares the three dimensional structure of the wild-type protease and a reference protease.
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