Method of cryopreserving hepatocytes
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United States of America Patent
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Oct 24, 2000
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N/A
app pub date -
Aug 2, 1999
filing date -
Apr 4, 1996
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Abstract
An artificial liver support system is described herein which comprises cryopreserved hepatocytes having an initial viability of 80-99%. Further disclosed are hepatocytes cryopreserved by dispensing hepatocytes into freezing containers, freezing the containers from between minus 50 to minus 90 degrees Celsius, storing the containers in liquid or vapor nitrogen, thawing the cryopreserved hepatocytes when ready for use and removing residual cryoprotectant media.
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